特(Te)别(Bie)提(Ti)示(Shi):(?)本(Ben)公(Gong)司(Si)的(De)所(Suo)有(You)产(Chan)品(Pin)仅(Jin)可(Ke)用(Yong)于(Yu)科(Ke)研(Yan)实(Shi)验(Yan),(?)严(Yan)禁(Jin)用(Yong)于(Yu)临(Lin)床(Chuang)医(Yi)疗(Liao)及(Ji)其(Qi)他(Ta)非(Fei)科(Ke)研(Yan)用(Yong)途(Tu)!(?)
产(Chan)品(Pin)名(Ming)称(Cheng) | 转(Zhuan)录(Lu)因(Yin)子(Zi)SOX10重(Zhong)组(Zu)兔(Tu)单(Dan)抗(Kang) | 抗(Kang)体(Ti)来(Lai)源(Yuan) | Rabbit |
英(Ying)文(Wen)名(Ming)称(Cheng) | SOX10 Recombinant Rabbit Monoclonal Anti | 浓(Nong)度(Du) | 1mg/ml |
规(Gui)格(Ge) | 25ul/50ul/100ul | 货(Huo)号(Hao) | EY-K16327 |
商(Shang)品(Pin)介(Jie)绍(Shao):(?)
别(Bie) 名(Ming):(?)DOM; MGC15649; SOX 10; SOX10_HUMAN; SRY (sex determining region Y) box 10; SRY box containing gene 10; SRY related HMG box gene 10; Transcription factor SOX 10; Transcription factor SOX-10; WS4.
研(Yan)究(Jiu)领(Ling)域(Yu)细(Xi)胞(Bao)生(Sheng)物(Wu) 免(Mian)疫(Yi)学(Xue) 神(Shen)经(Jing)生(Sheng)物(Wu)学(Xue) 干(Gan)细(Xi)胞(Bao) 细(Xi)胞(Bao)凋(Diao)亡(Wang) 表(Biao)观(Guan)遗(Yi)传(Chuan)学(Xue)
抗(Kang)体(Ti)来(Lai)源(Yuan):(?)Rabbit
克(Ke)隆(Long)类(Lei)型(Xing):(?)Recombinant
克(Ke) 隆(Long) 号(Hao):(?)26D7
交(Jiao)叉(Cha)反(Fan)应(Ying):(?)Human,Mouse,Rat
产(Chan)品(Pin)应(Ying)用(Yong):(?)WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理(Li)论(Lun)分(Fen)子(Zi)量(Liang):(?)50kDa
细(Xi)胞(Bao)定(Ding)位(Wei):(?)细(Xi)胞(Bao)核(He) 细(Xi)胞(Bao)浆(Jiang)
性(Xing) 状(Zhuang):(?)Liquid
浓(Nong) 度(Du)1mg/ml
免(Mian) 疫(Yi) 原(Yuan):(?)Recombinant human SOX10 protein (350-450aa)
亚(Ya) 型(Xing):(?)IgG
纯(Chun)化(Hua)方(Fang)法(Fa):(?)affinity purified by Protein A
缓(Huan) 冲(Chong) 液(Ye):(?)0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保(Bao)存(Cun)条(Tiao)件(Jian):(?)Shipped at 4℃(?). Store at -20℃(?) for one year. Avoid repeated freeze/thaw cycles.
注(Zhu)意(Yi)事(Shi)项(Xiang):(?)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产(Chan)品(Pin)介(Jie)绍(Shao):(?)This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate.
抗(Kang)体(Ti)的(De)多(Duo)样(Yang)性(Xing):(?)
抗(Kang)体(Ti)的(De)异(Yi)质(Zhi)性(Xing)。(?)抗(Kang)体(Ti)的(De)组(Zu)成(Cheng)极(Ji)为(Wei)复(Fu)杂(Za),(?)是(Shi)由(You)成(Cheng)千(Qian)上(Shang)万(Wan)、(?)多(Duo)种(Zhong)多(Duo)样(Yang)的(De)免(Mian)疫(Yi)球(Qiu)蛋(Dan)白(Bai)((?)Ig)(?)分(Fen)子(Zi)所(Suo)组(Zu)成(Cheng)。(?)这(Zhe)些(Xie)Ig分(Fen)子(Zi)在(Zai)形(Xing)状(Zhuang)、(?)大(Da)小(Xiao)、(?)结(Jie)构(Gou)以(Yi)及(Ji)氨(An)基(Ji)酸(Suan)的(De)组(Zu)成(Cheng)和(He)排(Pai)列(Lie)上(Shang),(?)既(Ji)相(Xiang)似(Si),(?)又(You)有(You)差(Cha)别(Bie)。(?)由(You)于(Yu)有(You)差(Cha)别(Bie),(?)它(Ta)们(Men)的(De)电(Dian)泳(Yong)活(Huo)性(Xing)就(Jiu)有(You)很(Hen)大(Da)的(De)变(Bian)化(Hua)。(?)
因(Yin)为(Wei)抗(Kang)体(Ti)具(Ju)有(You)与(Yu)抗(Kang)原(Yuan)决(Jue)定(Ding)簇(Cu)相(Xiang)对(Dui)应(Ying)的(De)结(Jie)合(He)部(Bu)位(Wei)((?)抗(Kang)原(Yuan)结(Jie)合(He)簇(Cu))(?),(?)所(Suo)以(Yi)抗(Kang)体(Ti)与(Yu)抗(Kang)原(Yuan)的(De)结(Jie)合(He)具(Ju)有(You)特(Te)异(Yi)性(Xing)。(?)另(Ling)一(Yi)方(Fang)面(Mian),(?)抗(Kang)体(Ti)本(Ben)身(Shen)是(Shi)一(Yi)种(Zhong)蛋(Dan)白(Bai)质(Zhi),(?)具(Ju)有(You)本(Ben)身(Shen)的(De)氨(An)基(Ji)酸(Suan)组(Zu)成(Cheng)、(?)排(Pai)列(Lie)和(He)立(Li)体(Ti)结(Jie)构(Gou),(?)对(Dui)异(Yi)种(Zhong)动(Dong)物(Wu)来(Lai)说(Shuo),(?)它(Ta)又(You)是(Shi)抗(Kang)原(Yuan)。(?)各(Ge)类(Lei)Ig都(Du)具(Ju)有(You)可(Ke)用(Yong)血(Xue)清(Qing)学(Xue)方(Fang)法(Fa)检(Jian)出(Chu)的(De)抗(Kang)原(Yuan)特(Te)异(Yi)性(Xing),(?)它(Ta)们(Men)表(Biao)现(Xian)出(Chu)不(Bu)同(Tong)的(De)血(Xue)清(Qing)学(Xue)类(Lei)型(Xing)。(?)
公(Gong)司(Si)正(Zheng)在(Zai)售(Shou)的(De)产(Chan)品(Pin):(?)
磷(Lin)化(Hua)皮(Pi)层(Ceng)肌(Ji)动(Dong)蛋(Dan)白(Bai)抗(Kang)体(Ti) | 转(Zhuan)录(Lu)因(Yin)子(Zi)相(Xiang)互(Hu)作(Zuo)用(Yong)蛋(Dan)白(Bai)1抗(Kang)体(Ti) |
Fas相(Xiang)关(Guan)磷(Lin)1抗(Kang)体(Ti) | 2×(?)YT培(Pei)养(Yang)基(Ji) |
干(Gan)扰(Rao)调(Diao)节(Jie)因(Yin)子(Zi)5抗(Kang)体(Ti) | 低(Di)限(Xian)肉(Rou)汤(Tang)((?)不(Bu)含(Han)葡(Pu)萄(Tao)糖(Tang))(?) |
磷(Lin)化(Hua)结(Jie)节(Jie)性(Xing)硬(Ying)化(Hua)症(Zheng)蛋(Dan)白(Bai)1抗(Kang)体(Ti) | SOC培(Pei)养(Yang)基(Ji) |
HIV-1病(Bing)毒(Du)复(Fu)制(Zhi)结(Jie)合(He)蛋(Dan)白(Bai)2抗(Kang)体(Ti) | 低(Di)限(Xian)琼(Qiong)脂(Zhi) |
锌(Xin)指(Zhi)蛋(Dan)白(Bai)224抗(Kang)体(Ti) | SOB培(Pei)养(Yang)基(Ji) |
腺(Xian)苷(Gan)环(Huan)化(Hua)激(Ji)活(Huo)肽(Tai)受(Shou)体(Ti)-II/血(Xue)管(Guan)活(Huo)性(Xing)肠(Chang)肽(Tai)受(Shou)体(Ti)-II抗(Kang)体(Ti) | TB培(Pei)养(Yang)基(Ji) |
促(Cu)代(Dai)谢(Xie)型(Xing)谷(Gu)氨(An)受(Shou)体(Ti)1抗(Kang)体(Ti) | 选(Xuan)择(Ze)性(Xing)APS肉(Rou)汤(Tang)基(Ji)础(Chu) |
细(Xi)胞(Bao)角(Jiao)蛋(Dan)白(Bai)31抗(Kang)体(Ti) | Luria琼(Qiong)脂(Zhi)基(Ji)础(Chu) |
卡(Ka)尔(?)曼(Man)综(Zong)合(He)症(Zheng)基(Ji)因(Yin)1抗(Kang)体(Ti) | Luria肉(Rou)汤(Tang)基(Ji)础(Chu) |
DNAJB11蛋(Dan)白(Bai)抗(Kang)体(Ti) | NZYM肉(Rou)汤(Tang) |
骨(Gu)骼(Ge)肌(Ji)快(Kuai)/慢(Man)肌(Ji)肌(Ji)钙(Gai)蛋(Dan)白(Bai)T抗(Kang)体(Ti) | NZCYM肉(Rou)汤(Tang) |
RNA结(Jie)合(He)蛋(Dan)白(Bai)6抗(Kang)体(Ti) | 转(Zhuan)录(Lu)因(Yin)子(Zi)SOX10重(Zhong)组(Zu)兔(Tu)单(Dan)抗(Kang)无(Wu)盐(Yan)离(Li)子(Zi)麦(Mai)康(Kang)凯(Kai)琼(Qiong)脂(Zhi) |
磷(Lin)化(Hua)c-fos抗(Kang)体(Ti) | Endo培(Pei)养(Yang)基(Ji) |
L(+)鼠(Shu)李(Li)糖(Tang) | EEM培(Pei)养(Yang)基(Ji) |
免(Mian)疫(Yi)荧(Ying)光(Guang)技(Ji)术(Shu)的(De)实(Shi)验(Yan)步(Bu)骤(Zhou):(?)
一(Yi)、(?)准(Zhun)备(Bei)好(Hao)试(Shi)剂(Ji)与(Yu)仪(Yi)器(Qi):(?)
磷(Lin)酸(Suan)盐(Yan)缓(Huan)冲(Chong)盐(Yan)水(Shui)、(?)荧(Ying)光(Guang)标(Biao)记(Ji)的(De)抗(Kang)体(Ti)溶(Rong)液(Ye)、(?)缓(Huan)冲(Chong)甘(Gan)油(You)、(?)搪(Tang)瓷(Ci)桶(Tong)三(San)只(Zhi)、(?)有(You)盖(Gai)搪(Tang)瓷(Ci)盒(He)一(Yi)只(Zhi)、(?)荧(Ying)光(Guang)显(Xian)微(Wei)镜(Jing)、(?)玻(Bo)片(Pian)架(Jia)、(?)滤(Lu)纸(Zhi)、(?)37℃(?)温(Wen)箱(Xiang)等(Deng)。(?)
二(?)、(?)实(Shi)验(Yan)步(Bu)骤(Zhou)
1.滴(Di)加(Jia)0.01mol/L,(?)pH7.4的(De)PBS于(Yu)待(Dai)检(Jian)标(Biao)本(Ben)片(Pian)上(Shang),(?)十(Shi)分(Fen)钟(Zhong)后(Hou)弃(Qi)去(Qu),(?)使(Shi)标(Biao)本(Ben)保(Bao)持(Chi)一(Yi)定(Ding)湿(Shi)度(Du)。(?)
2.滴(Di)加(Jia)适(Shi)当(Dang)稀(Xi)释(Shi)的(De)荧(Ying)光(Guang)标(Biao)记(Ji)的(De)抗(Kang)体(Ti)溶(Rong)液(Ye),(?)使(Shi)其(Qi)覆(Fu)盖(Gai)标(Biao)本(Ben),(?)置(Zhi)于(Yu)有(You)盖(Gai)搪(Tang)瓷(Ci)盒(He)内(Nei),(?)保(Bao)温(Wen)一(Yi)定(Ding)时(Shi)间(Jian)以(Yi)三(San)十(Shi)分(Fen)钟(Zhong)为(Wei)参(Can)考(Kao)。(?)
3.取(Qu)出(Chu)玻(Bo)片(Pian),(?)置(Zhi)玻(Bo)片(Pian)架(Jia)上(Shang),(?)先(Xian)用(Yong)0.01mol/L,(?)pH7.4的(De)PBS冲(Chong)洗(Xi)后(Hou),(?)再(Zai)按(An)顺(Shun)序(Xu)过(Guo)0.01mol/L,(?)pH7.4的(De)PBS三(San)缸(Gang)浸(Jin)泡(Pao),(?)每(Mei)缸(Gang)三(San)到(Dao)五(Wu)分(Fen)钟(Zhong),(?)并(Bing)不(Bu)停(Ting)地(Di)摇(Yao)晃(Huang)振(Zhen)荡(Dang)。(?)
4.取(Qu)出(Chu)玻(Bo)片(Pian),(?)用(Yong)滤(Lu)纸(Zhi)吸(Xi)去(Qu)多(Duo)余(Yu)水(Shui)分(Fen),(?)但(Dan)不(Bu)使(Shi)标(Biao)本(Ben)干(Gan)燥(Zao),(?)加(Jia)一(Yi)滴(Di)缓(Huan)冲(Chong)甘(Gan)油(You),(?)以(Yi)盖(Gai)玻(Bo)片(Pian)覆(Fu)盖(Gai)。(?)
5.立(Li)即(Ji)用(Yong)荧(Ying)光(Guang)显(Xian)微(Wei)镜(Jing)观(Guan)察(Cha)。(?)观(Guan)察(Cha)标(Biao)本(Ben)的(De)特(Te)异(Yi)性(Xing)荧(Ying)光(Guang)强(Qiang)度(Du)。(?)